Multifunctional magnetic Fe3O4 nanoparticles combined with chemotherapy and hyperthermia to overcome multidrug resistance
نویسندگان
چکیده
BACKGROUND Multidrug resistance in cancer is a major obstacle for clinical therapeutics, and is the reason for 90% of treatment failures. This study investigated the efficiency of novel multifunctional Fe(3)O(4) magnetic nanoparticles (Fe(3)O(4)-MNP) combined with chemotherapy and hyperthermia for overcoming multidrug resistance in an in vivo model of leukemia. METHODS Nude mice with tumor xenografts were randomly divided into a control group, and the treatment groups were allocated to receive daunorubicin, 5-bromotetrandrine (5-BrTet) and daunorubicin, Fe(3)O(4)-MNP, and Fe(3)O(4)-MNP coloaded with daunorubicin and 5-bromotetrandrine (Fe(3)O(4)-MNP-DNR-5-BrTet), with hyperthermia in an alternating magnetic field. We investigated tumor volume and pathology, as well as P-glycoprotein, Bcl-2, Bax, and caspase-3 protein expression to elucidate the effect of multimodal treatment on overcoming multidrug resistance. RESULTS Fe(3)O(4)-MNP played a role in increasing tumor temperature during hyperthermia. Tumors became significantly smaller, and apoptosis of cells was observed in both the Fe(3)O(4)-MNP and Fe(3)O(4)-MNP-DNR-5-BrTet groups, especially in the Fe(3)O(4)-MNP-DNR-5-BrTet group, while tumor volumes in the other groups had increased after treatment for 12 days. Furthermore, Fe(3)O(4)-MNP-DNR-5-BrTet with hyperthermia noticeably decreased P-glycoprotein and Bcl-2 expression, and markedly increased Bax and caspase-3 expression. CONCLUSION Fe(3)O(4)-MNP-DNR-5-BrTet with hyperthermia may be a potential approach for reversal of multidrug resistance in the treatment of leukemia.
منابع مشابه
Study on Fe3O4 Magnetic Nanoparticles Size Effect on Temperature Distribution of Tumor in Hyperthermia: A Finite Element Method
In recent years, Hyperthermia has been used as an emerging technique for cancer treatment, especially for localized tumors. One of the promising cancer treatment approaches is magnetic nanoparticle (MNPs) Hyperthermia. In this theoretical work, the temperature distribution of a common tumor over the different sizes of Fe3O4 magnetic nanoparticles, namely 25, 50, 100, and 200 nm, was stud...
متن کاملMagnetic mesoporous silica nanoparticles for potential delivery of chemotherapeutic drugs and hyperthermia.
Magnetic mesoporous silica (MMS) nanoparticles with controllable magnetization have been synthesized by encapsulating Fe3O4 nanoparticles in a mesoporous silica matrix. The structure, magnetic heating capacity and drug delivery ability of MMS nanoparticles were evaluated. The results showed that MMS nanoparticles had an average particle size of 150 nm and showed low cytotoxicity and efficient c...
متن کاملP(EO-co-LLA) functionalized Fe3O4@mSiO2 nanocomposites for thermo/pH responsive drug controlled release and hyperthermia.
The Fe3O4@mSiO2 nanocarrier that consisted of a magnetic Fe3O4 nanoparticle core and a mesoporous silica (mSiO2) shell was synthesized. It shows a uniform sphere morphology about 65 nm in diameter. Considering the magnetic hyperthermia of Fe3O4 under an alternating magnetic field (AMF), a thermo-sensitive polymer, poly[(ethylene glycol)-co-(L-lactide)] (P(EO-co-LLA)), was used as "gatekeeper" c...
متن کاملEffect of dispersion state of the magnetic Fe3O4 nanoparticles on the thermal distribution in Hyperthermia
Introduction: Magnetic nanoparticle (MNP) hyperthermia is a promising cancer treatment approach. It is based on the evidence that by injecting MNPs such as Fe3O4 in the tumor and subjecting them to an alternating magnetic field, they release heat, generating temperatures up to 42°C that can kill cancer cells by apoptosis, usually with lowest damage to normal tissue. In previous...
متن کاملMagnetic nanoparticle-based therapeutic agents for thermo-chemotherapy treatment of cancer.
Magnetic nanoparticles have been widely investigated for their great potential as mediators of heat for localised hyperthermia therapy. Nanocarriers have also attracted increasing attention due to the possibility of delivering drugs at specific locations, therefore limiting systematic effects. The enhancement of the anti-cancer effect of chemotherapy with application of concurrent hyperthermia ...
متن کامل